ISAS v1 has been developed as a system which gathers tools to be used in both clinic and research. Its application fields are human and veterinarian clinic, scientific research, livestock production and pharmaceutical and reproductive toxicology. It can be considered as the most complete and easy-to-use system in the market which, furthermore, works in different hardware and operating-system conditions, in order to be adapted, as much as possible, to the needs of our users.
ISAS v1 is a multi-specie system that can work with many different sperm samples: man, stallion, bull, boar, goat, rabbit, dog, rat, mice, rooster, mutton, cod, shark, tuna, anguilla and this list is continuously growing. It also has generic analysis configuration that allows sperm analysis of any specie.
ISAS v1 works with cameras up to 200 frames per second, allowing a near perfect track analysis. Furthermore, it is especially designed for quality control programs, allowing the analysis from video files of many different formats (WIndows AVI, Windows Media Video, MPEG-4, DVD, and many others) and images (jpg,png,bmp,gif,tif, ...). So you can have video and image collections for off-line analysis of mobility, concentration, morphology, vitality or DNA fragmentation.
The present equipment is completely modular, so each module might work both independently and jointly. Moreover, we have designed the system to allow you to use most of components you can have in your lab, from the microscope to the computer, if they fulfill the minimum requirements of the system.
Motility
ISAS v1 Motility performs the sperm motility and concentration analysis. It works in a very great range of species and each species has its own configuration and analysis algorithms.
Depending on the sample, both negative or positive phase contrast can be used. The motility analysis of one field is done in real-time (less than 1 second), and it can be configured to analyze motility up to 250 frames, at 200 frames per second and with concentrations higher than 1 000 sperms/field.
Aditionally to concentration , motility and progressivity percentages, following kinetic parameters are calculated: VCL , VAP , VSL , LIN , STR , WOB , ALH , BCF , DNC , MAD , ...
Morphometry
ISASv1 Morphometry performs the morphologic and morphometric spermatozoa analysis from
stained samples or fresh samples (using phase contrast optics). The morphometric analysis is done using different optics depending on species (40x, 60x or 1 00x) in short time.
ISASv1 Morphometry gives 14 morphometric parameters: head size (area, length, width and
perimeter), head shape (ellipticity, elongation, roughness, regularity), acrosome percentage, head
color level, midpiece size (area and width) and midpiece insertion (angle and distance).
DNA Fragmentation
ISASv1 DNA Fragmentation automatically performs the analysis of the percentage of DNA fragmentated sperm.
The fragmentation analysis of one field is done using 20x or 40x objectives in real-time (less than 1 second). Depending on the species, information about size of core and halo is used to define the DNA fragmentation level.
ISASv1 DNA Fragmentation requires the use of different kits, like Halotech DNA, based on the Sperm Chromatin Dispersion test (SCD) technology, or Tunnel Assay Test.
Vitality
ISAS v1 Vitality performs automatic viability analysis of sperm cells. We need to determine the real percentage of viability cells in a sample, and to perform accurately this analysis we need to use fluorescent techniques with their correspondent use of appropriate staining kits. Anyway, the classic eosine-nigrosine stain can also be used now!
Components
UB 203i
¬ Trinocular microscope
¬ Heated stage system
¬ Negative and positive Phase contrast
¬ Quintuple revolver nosepiece
¬ Power supply 110/220V, 50/60Hz, 5W.
¬ LED lamp 4W (equivalent to 50W halogen lamp)
¬ Reflected Fluorecence Attachment for vitality module or motility and concentration using Hoechst
PROISER 782
¬ Monochrome or color CCD camera
¬ Image sensor: 1/2" Progressive Scan CCD, SONY ICX415
¬ Resolution: 782x580, 8/12 bits per pixel
¬ Framerate: 63 fps at full resolution
¬ Regulations: FCC, CE, RoHS
¬ Dimensions: 44x29x63mm
¬ Power consumption: 260mA 12V DC (8VDC ¬ 30 VDC)
DELL OPTIPLEX
¬ Processor: Intel Core i3/i5
¬ Memory : 4GB (2x2GB) 1333MHz DDR3 NonECC
¬ Hard drive: 500GB 3,5" Serial ATA (7200rpm)
¬ Monitor 20"
¬ Windows 7/8 64 bits
ENGLISH
